Target Microorganism Selection

Our expert team leverages extensive scientific literature, databases, and advanced bioinformatics tools to identify the most relevant microbial candidates for targeted rare diseases.

Assay Format Selection

Various formats are available, including culture-based methods, molecular methods (PCR, qPCR), immunoassays (ELISA), and biosensor-based methods. The selection depends on factors such as the sensitivity required, the complexity of the sample, and the desired throughput.

Primer/Probe Design or Antibody Selection

Depending on the assay format, the next step involves designing specific primers/probes for molecular detection or selecting antibodies for immunoassays. The primers/probes or antibodies are designed to target unique sequences or antigens specific to the target microorganism.

Reagent Formulation and Optimization

Our scientists formulate assay reagents by designing and optimizing the components required for sample preparation, amplification (if applicable), detection, and analysis. Optimization includes adjusting reagent concentrations, buffer compositions, and reaction conditions to maximize the detection efficiency.

Performance Validation and Optimization

We use known positive and negative samples (including different strains or species of target microorganisms) for test evaluation, combined with statistical analysis, to validate the performance parameters of microbiological assays. Necessary adjustments are made to improve the sensitivity, specificity and accuracy of the test.

Kit Assembly and Quality Control

Finally, the assay components are assembled to create a complete reagent kit. Throughout this process, we implement stringent quality control measures to ensure consistency and reliability of products within the same batch.