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Antibody Detection Reagent/Kit Development Service

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Antibody Detection Reagent/Kit Development Service

Antibody detection plays a pivotal role in the diagnosis of rare diseases. With a professional talent team and advanced technology platforms, we focus on developing customized antibody detection reagents and kits to meet the specific needs of customers.

Antibody Detection Reagents/Kits for Rare Disease

Antibody detection reagents and kits play a crucial role in the diagnosis and research of rare diseases. These reagents and kits allow for the specific detection and quantification of antibodies associated with rare diseases.

Table. 1 Rare disease antibody detection reagents/kits available on the market

Reagent/Kit Target Detection Method
Neuromyelitis Optica (NMO) Antibody Detection Reagent/Kit Aquaporin-4 (AQP4) antibodies Indirect Immunofluorescence Assay (IFA)
Myasthenia Gravis (MG) Antibody Detection Reagent/Kit Autoantibodies against acetylcholine receptors (AChR) or muscle-specific kinase (MuSK) Radioimmunoprecipitation Assay (RIPA), ELISA
Lambert-Eaton Myasthenic Syndrome (LEMS) Antibody Detection Reagent/Kit Autoantibodies against voltage-gated calcium channels (VGCCs) Radioimmunoprecipitation Assay (RIPA), ELISA
Antiphospholipid Syndrome Antibody Detection Reagent/Kit Antiphospholipid syndrome-related autoantibodies ELISA, Chemiluminescent Immunoassay

Antibody detection system.Fig. 1 Antibody detection. (Kubo, Sousuke, et al., 2021)

Our Services

The development of antibody detection reagents/kits for rare diseases is a critical milestone in achieving accurate diagnosis and effective therapeutic monitoring. Leveraging our extensive expertise and advanced technologies, we can develop diagnostic reagents/kits that detect disease-specific antibodies, thereby aiding in the early diagnosis and classification of rare diseases.

Workflow of Antibody Detection Reagents/Kits Development

Selection of Target Antibody

The selection of target antibodies is the first step in kit development. Our team of experts has extensive expertise in the pathophysiology of rare diseases and related specific antibodies and can help you identify suitable target antibodies.

Antigen Design

In order to detect the target antibody, we need to design the antigen. The antigen should contain a receptor binding domain (RBD) that serves as the binding target for the antibody. The antigens can be obtained through recombinant protein expression, chemical synthesis, or isolation from biological samples.

Assay Format Selection

Depending on the specific requirements of the assays, we can choose detection methods such as ELISA, Western blot or immunofluorescence. We need to comprehensively consider factors such as sensitivity, specificity, dynamic range and sample throughput to make an appropriate choice.

Reagent Formulation

Reagent formulation involves selecting and optimizing various components such as detection antigens, blocking agents, buffers, and substrates. Our scientists carefully design and test each reagent component to ensure optimal performance, stability, and compatibility with the selected detection method.

Performance Verification and Optimization

At our company, we test the developed reagents with known positive and negative samples to evaluate their detection performance. We also use advanced statistical analysis to verify performance parameters and adjust and optimize the detection method in a timely manner.

Kit Assembly and Quality Control

Finally, by assembling the kit, a complete antibody detection kit is developed. Quality control measures are implemented at each stage of kit assembly to ensure batch-to-batch consistency, reliability, and quality.

Application of Antibody Detection Reagents/Kits

In the field of rare disease diagnosis, antibody detection reagents/kits play a significant role mainly in the identification of autoimmune diseases. These reagents/kits are designed to detect specific autoantibodies produced by the immune system that target the body's tissues or organs.

  • Autoimmune Encephalitis
  • Neuromyelitis Optica (NMO)
  • Myasthenia Gravis (MG)
  • Sjögren's Syndrome
  • Systemic Lupus Erythematosus
  • Antiphospholipid Syndrome (APS)
  • Dermatomyositis and Polymyositis
  • Celiac Disease
  • Goodpasture Syndrome
  • Autoimmune Hepatitis
  • Pemphigus and Pemphigoid
  • And More

Our Advantages

Time-saving services with high efficiency

Professional and experienced team

Cutting edge technology platform

Numerous service cases and customer praise

No matter which research stage you are at, we can provide comprehensive services to meet your needs. If you are interested in our services, please don't hesitate to contact us for further information and pricing details.

References

  1. Kubo, Sousuke, et al. "Development of an automated chemiluminescence assay system for quantitative measurement of multiple anti-SARS-CoV-2 antibodies." Frontiers in Microbiology 11 (2021): 628281.
  2. Farshchi, Fatemeh, and Mohammad Hasanzadeh. "Nanomaterial based aptasensing of prostate specific antigen (PSA): recent progress and challenges in efficient diagnosis of prostate cancer using biomedicine." Biomedicine & Pharmacotherapy 132 (2020): 110878.
  3. Kang, Jae Seung, et al. "Limits of serum carcinoembryonic antigen and carbohydrate antigen 19-9 as the diagnosis of gallbladder cancer." Annals of surgical treatment and research 101.5 (2021): 266.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.