Antibody Detection Reagent/Kit Development Service
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- Antibody Detection Reagent/Kit Development Service
Antibody detection plays a pivotal role in the diagnosis of rare diseases. With a professional talent team and advanced technology platforms, we focus on developing customized antibody detection reagents and kits to meet the specific needs of customers.
Antibody detection reagents and kits play a crucial role in the diagnosis and research of rare diseases. These reagents and kits allow for the specific detection and quantification of antibodies associated with rare diseases.
Table. 1 Rare disease antibody detection reagents/kits available on the market
Reagent/Kit | Target | Detection Method |
Neuromyelitis Optica (NMO) Antibody Detection Reagent/Kit | Aquaporin-4 (AQP4) antibodies | Indirect Immunofluorescence Assay (IFA) |
Myasthenia Gravis (MG) Antibody Detection Reagent/Kit | Autoantibodies against acetylcholine receptors (AChR) or muscle-specific kinase (MuSK) | Radioimmunoprecipitation Assay (RIPA), ELISA |
Lambert-Eaton Myasthenic Syndrome (LEMS) Antibody Detection Reagent/Kit | Autoantibodies against voltage-gated calcium channels (VGCCs) | Radioimmunoprecipitation Assay (RIPA), ELISA |
Antiphospholipid Syndrome Antibody Detection Reagent/Kit | Antiphospholipid syndrome-related autoantibodies | ELISA, Chemiluminescent Immunoassay |
Fig. 1 Antibody detection. (Kubo, Sousuke, et al., 2021)
The development of antibody detection reagents/kits for rare diseases is a critical milestone in achieving accurate diagnosis and effective therapeutic monitoring. Leveraging our extensive expertise and advanced technologies, we can develop diagnostic reagents/kits that detect disease-specific antibodies, thereby aiding in the early diagnosis and classification of rare diseases.
Selection of Target Antibody
The selection of target antibodies is the first step in kit development. Our team of experts has extensive expertise in the pathophysiology of rare diseases and related specific antibodies and can help you identify suitable target antibodies.
Antigen Design
In order to detect the target antibody, we need to design the antigen. The antigen should contain a receptor binding domain (RBD) that serves as the binding target for the antibody. The antigens can be obtained through recombinant protein expression, chemical synthesis, or isolation from biological samples.
Assay Format Selection
Depending on the specific requirements of the assays, we can choose detection methods such as ELISA, Western blot or immunofluorescence. We need to comprehensively consider factors such as sensitivity, specificity, dynamic range and sample throughput to make an appropriate choice.
Reagent Formulation
Reagent formulation involves selecting and optimizing various components such as detection antigens, blocking agents, buffers, and substrates. Our scientists carefully design and test each reagent component to ensure optimal performance, stability, and compatibility with the selected detection method.
Performance Verification and Optimization
At our company, we test the developed reagents with known positive and negative samples to evaluate their detection performance. We also use advanced statistical analysis to verify performance parameters and adjust and optimize the detection method in a timely manner.
Kit Assembly and Quality Control
Finally, by assembling the kit, a complete antibody detection kit is developed. Quality control measures are implemented at each stage of kit assembly to ensure batch-to-batch consistency, reliability, and quality.
In the field of rare disease diagnosis, antibody detection reagents/kits play a significant role mainly in the identification of autoimmune diseases. These reagents/kits are designed to detect specific autoantibodies produced by the immune system that target the body's tissues or organs.
Time-saving services with high efficiency
Professional and experienced team
Cutting edge technology platform
Numerous service cases and customer praise
No matter which research stage you are at, we can provide comprehensive services to meet your needs. If you are interested in our services, please don't hesitate to contact us for further information and pricing details.
References
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.